Solution-Enzymes and temperature, Describe the relationship

Exercise: Enzymes, Temperature, and pH

Data Table: Enzymes and Temperature.

Lactase Tube

Temperature (°C)

Time (minutes)

Glucose Concentration

 

 

5 minutes

100

Cold

5

10 minutes

100

15 minutes

100

20 minutes

100

Room

18

5 minutes

500

10 minutes

250

15 minutes

500

20 minutes

500

Hot

103

5 minutes

No change/Negative

10 minutes

No Change/Negative

15 minutes

No Change/Negative

20 minutes

No Change/Negative

A. Describe the relationship between temperature and the enzymatic activity of lactase. Of the three temperatures tested, which is the optimal temperature for enzyme activity? Use the results in Data Table to support your answer. Hypothesize how the structure of the lactase relates to the results in Data Table. At low temperatures, molecules move so slowly that reactions rates are hindered even though the enzyme is still folded and active.  At high temperatures, bonds and interactions that exist between the amino acid side chains in an enzyme become disrupted.  The enzyme is not able to keep its shape and unfolds and becomes inactive. It is now denatured.  The optimal temperature for enzyme activity is room temperature.  At room temperature the glucose concentration was at it’s highest.

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